Standardization of clinical enzyme assays.

Standardization of Assays That Detect Anti-Rubella Virus IgG Antibodies.

Rubella virus usually causes a mild infection in humans but can cause congenital rubella syndrome (CRS). Vaccination programs have significantly decreased primary rubella virus infection and CRS; however, vaccinated individuals usually have lower levels of rubella virus IgG than those with natural infections. Rubella virus IgG is quantified with enzyme immunoassays that have been calibrated aga...

Standardization of clinical enzyme assays: a reference method for aspartate and alanine transaminases.

Reaction rate measurement of multiple enzyme samples by continuous flow analysis.

A simple technic to adapt the advantages of continuous flow analysis to the kinetic assay of multiple enzyme samples is described. It will permit adequate standardization by primary or secondary standards run through the entire analysis of those procedures that do not exhibit spontaneous changes in light absorption or fluorescence with time. The adaptation of the Kind-King method for alkaline p...

Standardization in Clinical Enzymology – Results of a Survey Performed in 2008 in Big Hospital Laboratories in Poland

The survey was performed in December 2008 in 21 big hospital laboratories in Poland. The purpose of this survey was to estimate how the IFCC standardization in clinical enzymology is recognized and followed in medical laboratories. Each participant received a short questionnaire in an electronic version consisting of 5 questions dealing with the main features for the Reference Procedures for me...

An Alkaline Phosphatase Lacking Wheat Germ Agglutinin Binding Sites Useful Enzyme for Lectin Assays with Comparable Activity to the Calf Enzyme

Despite the availability of various alkaline phosphatase (ALP) isoenzymes, the calf enzyme is being used in current enzyme assays as the detector enzyme. The glycosylation pattern of this enzyme makes it a suitable ligand for binding to wheat germ agglutinin lectin (WGA). As a result of this property, the enzyme can not be used as a conjugate with this lectin, and the calf enzyme conjugates can...